What is the purpose of the spread plate technique. What is the purpose of the streak plate method? 2022-10-11

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A Farewell to Arms is a novel by Ernest Hemingway set during World War I. The story follows the experiences of an American ambulance driver, Frederick Henry, and an English nurse, Catherine Barkley, as they fall in love amid the chaos and devastation of war.

In Chapter 1, Henry is introduced as a young American man who is serving as an ambulance driver for the Italian army on the front lines of the war. He spends his days ferrying wounded soldiers to hospitals and attending to the needs of his comrades. Despite the constant danger and trauma of war, Henry finds some solace in his work and in the camaraderie of his fellow soldiers.

In Chapter 2, Henry meets Catherine Barkley, an English nurse working at a hospital in the town of Gorizia. Catherine is caring and compassionate, and Henry is immediately drawn to her. The two begin a tentative relationship, spending their days together when Henry is not on duty and talking about their hopes and fears for the future.

As the war rages on, Henry and Catherine's relationship deepens, and they become increasingly close. In Chapter 3, they decide to get married, even though they know that their future together is uncertain. They are married in a small ceremony at a chapel in the town of Treviglio, with only a few close friends in attendance.

In Chapter 4, Henry and Catherine's happiness is short-lived, as they are soon forced to flee the front lines as the Italian army retreats in the face of a fierce enemy attack. They flee to Milan, where they hope to find safety and wait out the war.

In Chapter 5, Henry and Catherine struggle to adjust to life in Milan, as they are separated from their friends and comrades and must deal with the constant threat of air raids and bombings. Despite these challenges, they try to make the best of their situation, taking comfort in each other's company and in the knowledge that they are at least safe from the horrors of the front lines.

As the war continues to escalate, Henry and Catherine's happiness is further tested by the arrival of a young Italian officer, Rinaldi, who is jealous of Henry's relationship with Catherine and tries to come between them. Despite Rinaldi's efforts, Henry and Catherine remain devoted to each other, and their love only grows stronger as they face the challenges of war together.

In the final chapter of the novel, Henry and Catherine must confront the ultimate test of their love as Catherine discovers that she is pregnant and becomes gravely ill. Despite his best efforts to save her, Catherine ultimately dies in childbirth, leaving Henry alone and devastated. In the aftermath of her death, Henry reflects on the fleeting nature of life and love and comes to understand the true meaning of his and Catherine's time together.

Overall, A Farewell to Arms is a poignant and powerful love story set against the backdrop of one of the most tumultuous periods in history. Through the experiences of Frederick Henry and Catherine Barkley, Hemingway explores the enduring human need for connection and the profound impact of war on the human psyche.

Spread Plate Method

what is the purpose of the spread plate technique

In other words, if the product is having too much bacterial count which is nearly uncountable , in that case also serial dilution is useful. In some situations a pour plate is better for working with antimicrobials, as it is essential to have time for a spread plate to dry fully before adding any other materials, such as paper discs soaked in antimicrobials. This ensures thinning of the bacteria with each streak, which ultimately produces isolated colonies after incubation. Comment on the practicality of agar as a solid culture medium for bacteria. The streak plate method does not require any additional media for dilution and only requires one plate for inoculation. The glass rod must be sterilized. Spread Plate Method The sample in the spread plate method must be liquid or in suspension.

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What is the purpose of the streak plate method?

what is the purpose of the spread plate technique

Therefore, it is a simple and easy method to perform in a lab. Crowding of the colonies makes the enumeration difficult. Furthermore, in streak plate, zig-zag pattern lines are drawn on the surface of the fresh medium while in spread plate, the inoculum is spread evenly on the surface of the medium. The number of colonies present on the plate is counted and multiplied by the dilution factor to calculate the total number of viable organisms initially present in the sample. The key difference between streak plate and spread plate is that the streak plate is used to isolate and purify a particular bacterial species from a mixture of bacteria while the spread plate is used to enumerate and quantify bacteria in a sample. Some individual bacterial cells are separated and well-spaced from each other. The glass rod is sterilized and used to spread the microbe-containing liquid uniformly on the plate.

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What is the purpose of the spread

what is the purpose of the spread plate technique

Also, it must not react to any component of the culture media. In pour plate technique, the process is to add the sample onto the solidified medium surface on the pour plate. What is the advantage of spread plate over pour plate? Count the number of colonies and multiply it by the appropriate dilution factor to determine the colony-forming units CFU present per ml in the original sample. What are the advantages 2 and disadvantages 3 of the serial dilution agar plate procedure? Bacteria is transferred to the edge of an agar plate with an inoculating loop and then treated out over the surface in one of several patterns. Summary — Pour Plate vs Spread Plate Pour plate and Spread plate are two techniques in microbiology to facilitate the enumeration of microbial cells in a sample. It can be used for all of the culturable bacteria and fungi.

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Why is spread plate method better than pour plate?

what is the purpose of the spread plate technique

A successful spread plate will have a countable number of isolated bacterial colonies evenly distributed on the plate. Furthermore, we can use an inoculating loop or cotton swab as an inoculating tool in streak plate while we need a spreader for spread plate technique. The spread plate method is a technique to plate a liquid sample containing bacteria so that the bacteria are easy to count and isolate. If in liquid, they are obligate anaerobes -aerotolerant anaerobes. The spread plate method is a microbiological laboratory technique for isolating and counting the viable microorganisms present in a liquid sample by spreading a certain volume of the sample over an appropriate solidified culture media.

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Why use spread plate method?

what is the purpose of the spread plate technique

The spread plate is ready to incubate. Care should be exercised in the opening of tubes with tight caps to prevent the breakage of the glass. Principle of Pour Plate Method In this Method, serial dilutions of the inoculum serially diluting the primary specimen are added within sterile Petri plates to which is poured melted and cooled 42-45°C agar medium and completely mixed by revolving the plates which are then left to solidify. In antimicrobial activity assay by diffusion using cross streak method involving two microbes, there would first be a streak of one of the microbe in the middle of the agar, and then, the other microbe s should be streaked perpendicularly to the first one. The spread plate method is a technique to plate a liquid sample containing bacteria so that the bacteria are easy to count and isolate.

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Difference Between Streak Plate and Spread Plate

what is the purpose of the spread plate technique

The loop is sterilized to reduce the number of bacteria being streaked on the plate. Embedded colonies are much smaller than those which happen to be on the surface. A culture of microbes can be diluted in the same fashion. A successful spread plate will have a countable number of isolated bacterial colonies evenly distributed on the plate. What is Spread Plate Method? What is the streak method? Spread plate technique is a viable counting method employed to plate a liquid sample for the purpose of isolating or counting the bacteria present in that sample.

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What are the advantages and disadvantages of spread plating?

what is the purpose of the spread plate technique

Streak plate technique is used to grow bacteria on a growth media surface so that individual bacterial colonies are isolated and sampled. How is spread plating used in medical research? Which plating method is best? Inoculum introduces into a fresh medium by drawing zig-zag pattern lines in streak plate while the inoculum spreads evenly throughout the surface in the spread plate. How is spread plate culture used to isolate bacteria? The technique makes it easier to quantify bacteria in a solution. Then, pass it briefly through the flame of a Bunsen burner and carefully allow the ethanol to burn off. The key difference between Pour plate and Spread plate is that a known volume of the sample is spread on the surface of the agar medium in spread plate, while, in pour plate, a known volume of the sample is mixed with agar and then poured into a plate. It is useful for the samples having heat sensitive microbes.

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What is the advantage of spread plate over pour plate?

what is the purpose of the spread plate technique

To sterilize a plate spreader, first douse it in ethanol. Quadrant Streak Plate Technique Purpose: 1. Organisms will stay clumped together and harder to identify the colonies. If plastic disposable loops are being utilized, they are removed from the packaging to avoid contamination and after being used, are discarded into an appropriate container. In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria.

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