Unknown bacteria lab report. (Sample) Unknown Lab Report 2022-10-14
Unknown bacteria lab report
An unknown bacteria lab report is a document written to identify and describe a previously unidentified bacteria sample. The purpose of the report is to accurately identify the bacteria and to describe its characteristics, including its appearance, behavior, and any special features that may be relevant to its identification.
To begin the process of identifying an unknown bacteria sample, a series of tests and observations must be performed. These may include tests to determine the bacteria's shape, size, and arrangement, as well as tests to determine its biochemical and physiological characteristics. For example, a gram stain test may be performed to determine the type of cell wall present in the bacteria, and a catalase test may be used to determine whether the bacteria produces the enzyme catalase.
Once the results of these tests have been analyzed, the bacterium can be tentatively identified based on its characteristics. However, it is often necessary to confirm the identification through additional testing, such as using a biochemical identification system or performing a series of specialized tests.
Once the bacterium has been fully identified, the lab report should include a description of the bacteria's appearance and any relevant characteristics, as well as a discussion of the tests and observations that were performed in order to identify it. The report should also include any relevant information about the bacterium, such as its natural habitat, any known pathogenic properties, and any other relevant information.
In conclusion, a unknown bacteria lab report is a critical document that allows scientists to accurately identify and describe previously unknown bacteria samples. By carefully performing a series of tests and observations, and by thoroughly documenting the results of these tests, scientists can gain a deeper understanding of the characteristics and behaviors of different types of bacteria, which can be invaluable in a variety of fields, from medicine to environmental science.
Isolation of 2 Bacteria
With these results, I narrowed down the possible species to Enterococcus faecalis, Enterococcus faecium, Lactococcus lactis, Leuconostoc mesenteroides, Pediococcus parvulus, and Streptococcus mitis. Below is a table which gives the name, principle, procedure, and results of all the tests I performed. Using an inoculating loop, streak the organism onto a NA plate for colony isolation and incubate at 37 degrees Celsius 25 degrees Fahrenheit for 24 to 48 hours. The proper procedure for all of these tests can be found in the laboratory manual. Being able to determine the identities of microorganisms is a very important task that can help people in many different settings. By going through the steps to identify the microorganism, students began to comprehend infectious disease. This test was done in accordance with the procedure that is described on page 200 in the lab manual.
How do you write an unknown bacteria in a lab report?
Table 2 lists the test, purpose, reagents and results for culture 2. Explain in detail about your further proceedings of the experiment? After isolating the second bacteria, a Gram stain was performed, hoping to see a purple color, indicating a gram positive. After removal with bacteria on the loop, the quadrant streak method was used. However, not all E. First, a drop of culture was aseptically transferred to a slide and dried.
(Sample) Unknown Lab Report
It is important to inform physicians of the identification characteristics of the bacteria in order for the physician to start the appropriate treatment as soon as possible. The goal was to demonstrate how to Gram staining, endospore staining, use MIO tubes, NA, EMB, and MSA plates to classify the unknown bacterias. UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. To determine this we recorded the results of how the bacteria reacted to different media. Louis Community College, 2011. Results Morphology Streptococcus Morphology Singular Rod Gram-positive Hemolytic Beta Gram-negative EMB Urease +++, green sheen Catalase - Bile- esculin Citrate - For the gram-positive cocci species, the tests allowed the identification of a bacteria that is biochemically unable to produce catalase, unable to live in the presence of bile, and highly able to lyse red blood cells. Fourth, make sure that all utensils and dishes used to cook are cleaned properly.
Lab Report Example: Classification of the Unknown Bacterias
No red ring formation after 15 to 20 minutes is a negative result. The Gram stain showed it was Gram positive rods. Marine Biologist preserve specimens and samples of unknown species and diseases and mapping the distribution, ranges or movements of marine population, designing scientific experiments and collating findings, preparing detailed reports for agencies, commercial organisation, communicating the latest advances in marine science to help improve Unknown Lab Report Essay Name: Danielle Title: Unknown Lab Report Introduction: There are many reasons for identifying an unknown bacterium. Professor Snaric advised to do another MSA agar from the original stock, but this time with a sterile swab instead of an inoculating loop. A change in the color to red indicates urea degradation. Upon reviewing the identification tables, the deciding biochemical test was the Casein test which tests for the production of the enzyme casease to break down the milk protein casein. A sample was taken from this colony and transferred to a nutrient broth agar to further isolate it.
Unknown Bacteria: Microbiology Lab Report
Multiple procedures were practiced to determine how bacteria thrive and grow in a laboratory setting. Second, people should know that pasteurized drinks are safer than unpasteurized. Identifying bacteria can lead to many new discoveries such as new species or the evolution of a known species. This is important in the medical field because identification of unknown Materials and Methods A mixed culture of two unknown bacteria was provided by the instructor. Using differential media, the unknown was confirmed to be a glucose, lactose, and sucrose.
How to Write a Microbiology Unknown Lab Report
Then it was important to record the number of the unknown bacterium as well as the temperature at which the bacteria would be incubated. The outcome of these tests indicated that unknown is Enterococcus faecalis. The gram stain procedure was performed as directed in the lab manual 1. As well as using the quadrant method to further isolate the colonies, a sample was taken from the best colony on the original streak and gram stained. There are several sections to this lab. A nutrient broth inoculated with bacterial culture numbered 45, henceforth referenced as U45 was selected and a streak plate was made to isolate a pure culture for use throughout the assignment.
Unknown Bacterial opportunities.alumdev.columbia.edu
Ultimately, after the last test, the MacConkey test, the clear organism that emerged was Klebsiella pneumoniae, for this organism was the only lactose fermenter that was left in the group following the previous test. After successful isolation of both bacteria, there were no more issues encountered in identifying either. Berg, 2004 If a motility test is done the tube would be cloudy indicating the bacteria is motile. It is most dangerous to cystic fibrosis patients and is involved and complicates 90% of cystic fibrosis deaths. Materials and Procedures The unknowns that were tested were numbers 48 and 74. Unfortunately, another issue arose. While this is helpful in a classroom setting, this luxury is not always present in the real world.
Example of a Microbiology Unknown Lab Report
Each experiment served to help eliminated and match the unknown culture to a culture worked with in a lab. Increases cells affinity for the stain c. This bacteria can catalyze in many environments which makes it very common and found almost everywhere such as soil, water, humans, plants, sewage and hospitals. Bacteria that are able to esculetin to utilize the esculin will turn black due to the reaction between ferric citrate and esculetin. Identification of bacteria is important because they are not only useful but potentially dangerous as well.