Microbiology lab report example. Example of Unknown Lab Report, Microbiology 2022-10-16
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Microbiology labs study microorganisms such as bacteria, viruses, fungi, and parasites. These tiny organisms play important roles in various fields including medicine, food science, and environmental science. In a microbiology lab, various techniques are used to identify and characterize these microorganisms.
A typical microbiology lab report includes several sections. The first section is the title, which should be concise and informative. The next section is the introduction, which provides background information about the microorganism being studied and the purpose of the experiment.
The materials and methods section describes the specific techniques used in the experiment, including the type of media used for culturing the microorganisms and the methods for isolation and identification. It is important to provide enough detail in this section so that the experiment can be replicated by others.
The results section presents the findings of the experiment in a clear and concise manner. This may include tables, graphs, and microscopy images. It is important to accurately report the results, including any unexpected findings.
The discussion section interprets the results and places them in the context of the research question being addressed. This section may also include a comparison of the results to previous studies on the same or similar microorganisms.
The conclusion section summarizes the main findings of the experiment and their implications. It may also include suggestions for future research.
In addition to these sections, a microbiology lab report may also include a list of references citing the sources used in the experiment.
Overall, a microbiology lab report is a detailed document that communicates the methods, results, and conclusions of an experiment studying microorganisms. It is an important tool for sharing research findings with the scientific community and advancing our understanding of these tiny but important organisms.
Example of Microbiology Unknown Lab Report
The enzyme extracellular protease is important in the virulence of these bacteria because it helps it adhere and invade its victims. Antibodies linked to large particles such as latex beads can be used to agglutinate microorganisms using specific reactive antibodies. The enzyme catalase degrades the hydrogen peroxide in the cell before it can do any cell damage. The Petri dish with colonies between 15 and 300 was selected to be significant in number whereas Petri plates with TMTC too many to count were not considered to be significant. With more tests confirming the identity of an organism we can more reliably confirm the identity of the organism in question.
Discussion Normally it is very difficult to determine the actual number of microorganisms in a population. Finally a clear gram negative with rods has appeared on the slide. Before starting the unknowns project our group performed a knowns project, which means we knew which organism we had each individual had their own organism and we performed several tests on them and recorded the results as well as write a description if it was necessary. I placed the prepared slide with my blood on the microscope stage, and brought it into focus on low power. .
. This is the case with our other tests and is the basis for making a flow chart to identify our unknown organism. Escherichia coli colony morphology and microscopic appearance, basic characteristic and tests for identification of E. Next, I did the gelatin hydrolysis test which tests for the enzyme gelatinase. They do have a merit that is useful in the daily life of people Booth, 2000.
It does not allow gram positive bacteria to grow and indicates lactose fermentation by the color of the colony. Only organism able to live in the presence of bile will grow on the bile enriched plate. Most sections of the lab report should be written in the past tense since it describes scientific work that has already been completed. Check through the rubric to find out the exact specifications that your instructor has requested regarding length, formatting, margins, font type and size, and writing style. The main portion of this manual involves the identification of unknown microbial agents.
Introduction: Bacteria are ubiquitous in nature. Use bold type to make these obvious. After many contaminated gram stains the gram negative was given by the instructor in a test tube. Various pH indicators are often added for differentiation of microbes based on their biochemical reactions. If the smear is too thick, it can also be difficult to recognise the decolourisation of the Gram negative bacteria6. Knowing the morphology, biochemistry, and ecology of all the different microbes can be useful in diagnosis when the proper tests have been performed to determine the organism. However, it takes some time for the visible colonies to grow Gerard J.
Six Petri plates are labeled 10-2 through 10-7. The results for the Gram negative bacterium after tests were performed was identified as Klebsiella pneumonia. Different nutrients may be added to the medium, making it higher in protein or in sugar. The tests we use to identify our organism tell us how a specific bacterium reacts biochemically in a given environment its surrounding nutrients and how it utilizes it, i. Material obtained from another source should be referenced. Acidic conditions, pH below 6, turns the medium green. This is because there are more microorganisms in the dilution of 10-2, overcrowded occurred, thus, less visible colonies can be grown on the agar plate due to competition among each other.
Enterobacter aerogenes is a Gram-negative rod-shaped bacterium belonging to the Enterobacteriacease family. This species is also responsible for the bad odor someone gets from sweating and not wearing deodorant because M. The bacterium might not have mixed well with the Glycerol solution, creating an improper answer. Acid-fast cells, due to the mycolic acid, bind with the primary stain much more easily and resist decolorization much more effectively. Include results for all dilutions, even if they are too numerous to count TNTC or 0. Gram staining separates bacteria into two groups dependant on their cell wall composition5. The immune system makes proteins called immunoglobulins or antibodies which bind to an antigen to either directly neutralize the antigen or cause it to be cleared more efficiently by other components of the immune system.
In hospitals, it is used to separate bacteria from DNA samples of patients to determine what infection they may have. There are two different arrangements of the shape shown, in chains as streptococci , and in clusters as staphylococci. This includes the potential implications of the research, and possibilities for future research that would contribute more to the field. The in-text reference to a table or figure should not repeat the caption e. If a plate has more than 300 colonies record it as TNTC too numerous to count. .
. This report assessed the growth, morphology characteristics, differential colony features of the four selected microorganisms E. Among the methods of enumeration, viable plate counts are being used most frequently to measure bacterial populations. If not, then it will remain solid and become solid when cooled down if it liquefied due to what I could of those onto the negative side of the capsule stain. I viewed the specimen under the microscope.
This is a differential stain that tests for mycolic acid in the cell wall. It is best to write your first draft and then let it sit for a few days before you read it the next time. In the second experiment, pre-cooked food is observed for faucal contamination. Most laboratory courses will require that data be immediately written into a lab notebook in pen. The outcome of the Urea test was positive, because the color of the broth changed to pink. Method First, we prepared an agar plate. See our course calendar for online registration or contact us directly by phone, live chat or email with questions.