Example of unknown bacteria lab report. Final Lab report of BIO 265 Unknown microbe experiment 2022-10-22

Example of unknown bacteria lab report Rating: 9,3/10 1018 reviews

An unknown bacteria lab report is a document that describes the process of identifying an unidentified bacterial sample. This type of report is typically used in microbiology labs, where scientists and students are often tasked with identifying unknown bacteria as part of their coursework or research.

To identify an unknown bacterial sample, a number of techniques and tests must be used. These techniques can include physical and chemical characteristics of the bacteria, as well as its growth patterns and ability to survive in different environments.

One example of an unknown bacteria lab report might involve the identification of a bacterial sample that was collected from a patient with a respiratory infection. The sample would be grown in a laboratory setting and observed for its physical characteristics, such as shape, size, and color. The sample might also be tested for its ability to survive in different temperatures, pH levels, and other conditions.

To further identify the bacterial sample, the lab might use biochemical tests to determine the presence or absence of certain enzymes or other chemical compounds. For example, the lab might test the sample for the production of certain enzymes that are characteristic of certain bacterial species, such as lactase or urease.

Once all of the data has been collected and analyzed, the lab can use a combination of physical, chemical, and biochemical characteristics to identify the unknown bacterial sample. The lab report would then include a detailed description of the identification process, as well as any relevant information about the identified bacteria, such as its potential to cause disease and how it might be treated.

In conclusion, unknown bacteria lab reports are an important tool for identifying and understanding the characteristics and behavior of different bacterial species. These reports provide valuable information that can be used to better understand and treat bacterial infections, and they are an essential part of the scientific process.

Microbiology Unknown Lab Report

example of unknown bacteria lab report

Always wash vegetables and fruits before eating or preparing them. It is quite common in nature and has also been attributed as part of the human intestinal flora. It looked over grown, so the same nutrient agar test was carried out several times. Several nutrient agar plates where inoculated and two different bacteria never grew. A gram positive rod was the outcome, ruling out the gram positive cocci. This species is also responsible for the bad odor someone gets from sweating and not wearing deodorant because M. It is most commonly found in mammals intestines.

Next

Unknown Bacteria: Microbiology Lab Report

example of unknown bacteria lab report

After running the sequence through the BLAST analysis function on the NCBI page, I received confirmation that my unknown was E. After heat fixing the smear on a slide, crystal violet was applied for one minute. The organism did not have a halo surrounding it, which means it was a negative result refer above to when I explain how the capsule stain works See Figure 2. The gram stain procedure was performed as directed in the lab manual 1. INTRODUCTION The identification of an unknown is beneficial in many ways, including the identification of bacteria in diseases, and in research. Following the previous test, an Indole test was performed via a SIM Sulfur Indole Motility test.

Next

unknown bacterium lab report

example of unknown bacteria lab report

His study picked up on the unique blue-green pigmentation of P. At this point I was able to confidently conclude that the Gram-positive bacteria that I isolated from my oral cavity was indeed S. The unknown gram negative bacteria had a negative reaction verifying that the unknown bacteria is Escherichia coli. Both of these organisms are important in one respect or another. The urea broth was inoculated and incubated. If the citrate is being utilized, basic conditions are produced. The sucrose test was chosen to distinguish between both species of Bacillus and as it can be observed in Figure 5, it gave a negative result, meaning that our unknown sample A is, presumably, B.


Next

Example of an Unknown Lab Report in Microbiology

example of unknown bacteria lab report

In order to do this, I first had to isolate DNA from the bacteria by lysing the cells. Bacteria are ubiquitous, free-living organisms often consisting of one biological cell. I confirmed this was the Gram-positive bacteria with the gram stain. MATERIALS AND METHODS The lab instructor gave out an unknown labeled 124, which contained a gram positive bacterium and a gram negative bacterium. Currently, the CDC is tracking an outbreak associated with ground beef that is caused by E. In order to isolate a Gram positive and Gram negative from myself, I took samples from several different parts of my body, specifically the oral cavity, ear canal, armpit, gastrointestinal GI tract, and the back of the ear. This led to the Nitrate test with a positive result that narrowed it down to four bacteria.

Next

Example of Unknown Lab Report, Microbiology

example of unknown bacteria lab report

Lastly, after another water rinse, safranin was added to the slide for one minute and then rinsed and dried. The goal was to demonstrate how to Gram staining, endospore staining, use MIO tubes, NA, EMB, and MSA plates to classify the unknown bacterias. For a flow chart to be successful, it is essential that the individual find a difference between all the organisms, and then find a difference between the organisms that diverged on the left as well as the right, etc. Even so, in the clinical setting, in which speed is paramount, biochemical tests coupled with other immunological techniques are often the preferred way to quickly assess whether a known specific bacterium is present or absent in a sample. The next test performed was a Nitrate Test which gave a positive result. It was a positive result. Scientist use Bacillus subtilis in order to promote genetic research and due to its highly genetic manipulability levels, it is easier to conduct testing on Swartzburg, 2009.

Next

(Sample) Unknown Lab Report

example of unknown bacteria lab report

The cells appear cocci-shaped and formed grape-like clusters; these gatherings led to the conclusion that the organism is in a Staphylococcus arrangement. Gives all cells a purple colour b. Then a urea test was performed to check for the production of urease. To confirm the identity of sample A, the BEA, nitrate and MR tests were performed. Figure 4: the oxidase testPositive result for Figure 5: for the sucrose testNegative result A B C Sample B Afterwards, by both visualizing the sample under the microscope and by performing a Gram stain, it was possible to conclude that the unknown is a rod-shaped, Gram negative bacterium Figure 7A.

Next

Unknown Microbiology Report Sample

example of unknown bacteria lab report

After doing Gram stain on this bacterium and concluding it was gram negative rods, the EMB test was used to narrow the spectrum. Next, the MacConkey and EMB tests were performed to detect if the bacterium was an enteric and fermenter of lactose. Try to avoid unpasteurized dairy products. A nitrate broth was inoculated and incubated, afterwards reagent A and B were added to the solution and did not turn red so then zinc was added to the solution and the broth turned red meaning the nitrate did not reduce to nitrite. An Eosin Methylene Blue Agar plate was used and the results came back as positive.

Next

Unknown sample Lab Report opportunities.alumdev.columbia.edu

example of unknown bacteria lab report

UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. This style abbreviates the titles of journals. For this test, the positive control was S. Verifying the gram negative bacteria is Escherichia coli. Microorganisms have much longer history than that of mankind.

Next

Unknown project lab report 4

example of unknown bacteria lab report

The first biochemical test performed was a negative Urea test which left two bacteria. There is only Gram positive bacterium grew on the nutrient agar. The purpose of this test was to determine whether or not the bacterium could utilize maltose as its sole carbon source McDonald et al. With the completion of this test, Escherichia coli was confirmed as the unknown Gram negative bacterium. The importance of identifying microorganisms is essential to the survival of humans, expansion of modern day medicine and improvement of quality of life. Because of the extensive research I accomplished, I have come to determine that the unknown bacterium 12C is Staphylococcus epidermidis.

Next