Drosophila melanogaster lab report. Drosophila melanogaster Fruit fly Report Sample Essay Example 2022-10-31
Drosophila melanogaster lab report
Drosophila melanogaster, also known as the fruit fly, is a commonly used model organism in genetics and molecular biology. It has a short lifespan, high fecundity, and a simple genome, making it an ideal subject for laboratory study. In this lab report, we will describe a study in which Drosophila melanogaster was used to investigate the genetic basis of a trait.
The specific trait under investigation in this study was bristle number, which refers to the number of hairs or bristles on the body of the fly. This trait is known to be under genetic control, and previous research has identified several genes that play a role in bristle development. In this study, we sought to identify any additional genes that might be involved in the regulation of bristle number.
To conduct the study, we first set up a breeding population of Drosophila melanogaster in the laboratory. We allowed the flies to mate and produce offspring, and we kept track of the number of bristles on the bodies of the parent flies and their offspring. We then performed a statistical analysis to identify any correlations between bristle number and the genetic makeup of the flies.
Our analysis revealed several genes that were significantly associated with bristle number. One of these genes, called "gene X," appeared to have a particularly strong effect on bristle number. To confirm the role of gene X in bristle development, we conducted a series of experiments in which we genetically modified the flies to either knock out or over-express gene X. We found that flies with a mutated or absent copy of gene X had significantly fewer bristles compared to wild-type flies, while flies with an over-expressed copy of gene X had significantly more bristles.
These findings suggest that gene X plays a critical role in the regulation of bristle number in Drosophila melanogaster. Further research will be necessary to fully understand the mechanisms by which this gene influences bristle development, and to determine whether it has any other functions in the fly.
In conclusion, this study demonstrates the utility of Drosophila melanogaster as a model organism for genetic studies. By using this simple and well-understood species, we were able to identify a gene that plays a key role in the regulation of a specific trait. This information will be valuable for future research on the genetics of bristle development, as well as for other studies on the genetic basis of other traits in Drosophila melanogaster.
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Additionally, his Law of Independent Assortment states that different alleles that are passed from first had to undergo a process of of being anesthetized. New labelled vials were set up for the F2 generation. The drosophila egg is about half a millimeter long. These flies breed rapidly in cycles of approximately 10 days and are extremely undemanding in terms of laboratory maintenance. We will be using Drosophila to examine patterns of inheritance over the course of the next several labs. As the pupa undergoes this transformation within the puparium, the cuticle of the exoskeleton hardens and darkens. An equal amount of F1 males and females were added to the vials.
Drosophila Cross Lab Report: Comparing the Given Genotype to the Phenotype
The expected ratio was 1:1 ruddy to white. Seven to ten yearss subsequently legion larvae and some pupae became seeable inside the bottle. F2 Reciprocal Cross data summary Reciprocal Cross Phenotype: Male Female Fly Count Red eyes and wild wing 45 112 157 White eyes and wild wing 83 51 134 Red eyes and apterous 3 8 11 White eyes and apterous 9 4 13 Totals 140 175 315 Table 15. The expected ratio of the void hypothesis was 3:1 75 % red-eye colored and 1:3 25 % sepia-eye colored or expected Numberss of 268. In his study he concluded that ebony flies reproduced much more thoroughly if they remained in the dark throughout their lifespan.
Drosophila melanogaster lab report
Along with sex-linkage, epistatic inheritance occurs when multiple genes affect a single phenotype such as eye-color Klug, 2014. Female Drosophila are XX, and males XY. Rendel also discovered a possible preference of ebony flies over vestigial flies when wildtype flies mate; however, he does not have a hypothesis for why this might be Rendell. The cuticle of the 3rd instar hardens into a pupa, and after 6 days in the puparium, metamorphosis is complete and the adult fly forces its way through the anterior end of the puparium. Quickly, the abdomen darkens and the wings expand. BASIC GENETICS The karyotype of Drosophila comprises four pairs of chromosomes, of which three pairs are autosomes and one pair are sex chromosomes.
Drosophila melanogaster lab report
We can conclude this considering our F1 generation ratio between male and female flies were closer to equal. Since a female fruit fly has two X chromosomes, she will obtain two copies of each X linked gene. The virgin females were placed inside an empty food vial, then the males were added. . The adults were cleared from the vials when the new pupal cases appeared. Over the next four days, the body is completely remodeled to give the adult winged form, which then hatches from the pupal …show more content… Some larva containing vials had hatched into flies. I have sincerely enjoyed this lab.
Drosophila melanogaster Fruit fly Report Sample Essay Example
The staying 25 7 % were of the sepia-eye coloured phenotype with a definite genotype of homozygous rr. A small paintbrush was used to carefully pick up the fly while anesthetized and put it into the vial once looked at. The roles fruit flies play in genetics also help the understanding of genetics in humans and other animals. Normal wings Normal wings No wings white No wings Red eyes White Drosophila Melanogaster Lab Report Identifying unknown phenotypes and determining mechanisms of inheritance of various genes in mutations of Drosophila melanogaster Michael Fisher, Tori Hall, Lindsey Theodore Crosses carried out with Drosophila melanogaster can be used to determine mechanisms that modify ratios of Mendelian inheritance, such as autosomal dominant and recessive inheritance, genetic linkage, and epistasis. The result of the coefficient of selection should be at the rate between 0 and 1. The expected chance ratios were theoretically supposed to hold closely mimicked the experimental consequences ; nevertheless chance is merely that. Finally, because fruit flies are insects, there are no laws about testing them in laboratories unlike other animals Dow, 2012.
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A male has only one X chromosome that is paired with a Y. A reason could be that the flies being identified for phenotype could have been looked at incorrectly or written down wrong. Gene, locus, and allele are often used more or less interchangeably, and this can lead to confusion. Additionally, their simple genome makes recognizing traits and analyzing inheritance especially easy compared to other organisms. After the larval stages, the fly then takes form of a pupa, which develops into the mature adult fly GSA, n.
Drosophila Melanogaster Lab Report
Discussion The primary focus of the Drosophila lab was to perform crosses of flies to determine if the chromosomal theory of inheritance that Thomas Hunt Morgan proposed, was true. A record was kept on the information needed for the F1 generation flies regarding sex and phenotype for each cross. The flies were carefully shaken to the bottom of the vial by tapping it, then quickly the plug was removed and the flies were transferred into a clean vial. There were some Works Cited Genetics Generation. Sex-linked Cross:A civilization bottle with Drosophila media and barm was prepared and labeled.
Drosophila Melanogaster Lab Report Essay
The intent of our D. The Genes We Share. A heterozygous female will always display the dominant wild-type Determining Sex Aside from determining eye colour, recognising the sex of D. Many different species, and a large number and wide variety of naturally-occurring and artificially-induced genetic variants are available. A female fruit fly will lay between 750 to 1,500 eggs in her lifetime.
M Rendel provided some evidence of both of these mutations affecting the reproduction patterns of the mutated flies. Drosophila melanogaster is inexpensive to rear, and can survive easily on food made of a starchy substance such as potato flakes or cornmeal, agar, yeast, and a mold inhibitor. The flies can have white, red, or sepia colored eyes, the bodies can be ebony or grey, and the wings can be long, short, or wingless. Many reasons are that they have a rapid reproduction rate, easy to care compared other animals and less expensive. Introduction In most kitchens the small flies that are found are Drosophila Melanogaster also called fruit fly.
DecisionThe consequences of both of these experiments were similar in footings of the credence or rejecting of the void hypothesis. This was then used to retrospectively determine the genotype of the F1 generation. A new generation is produced every week, allowing research to proceed quickly with little time for contamination from the environment. This means that even though there are outlooks for the consequences. As flies started to grow, at different rates for each vial, with in the first seven days after all larva had hatched the flies were counted. He reported different wing patterns and, by 1908, had noticed the proliferation of dwarf mutants.