Analysis of vitamin c in fruit juice. Analysis of Vitamin C in Fruit opportunities.alumdev.columbia.edu 2022-10-14
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Vitamin C, also known as ascorbic acid, is a water-soluble nutrient that is essential for human health. It is a powerful antioxidant that can protect cells from the damage caused by free radicals, which are unstable molecules that can damage cells and contribute to the development of chronic diseases such as cancer and heart disease. Vitamin C is also important for the synthesis of collagen, a protein that helps to form the structure of skin, blood vessels, and connective tissue. In addition, vitamin C plays a role in the immune system, helping to protect the body against infections.
One way to ensure that you are getting enough vitamin C is by consuming fruit juice, which can be a convenient and tasty source of this nutrient. However, it is important to choose a fruit juice that is high in vitamin C to get the maximum benefits. Some fruit juices that are particularly high in vitamin C include orange juice, grapefruit juice, and pineapple juice.
To analyze the vitamin C content of fruit juice, a sample of the juice is first prepared by diluting it with water to a known concentration. This sample is then placed in a test tube or other container and a reagent, such as 2,6-dichlorophenolindophenol (DCPIP), is added. The DCPIP reacts with the vitamin C in the juice, causing a color change that is proportional to the concentration of vitamin C present. The intensity of the color change can be measured using a spectrophotometer, which is a device that measures the amount of light absorbed by the sample. The results can be expressed as a percentage of the vitamin C content in the juice.
Another method for analyzing vitamin C in fruit juice involves the use of a titration. In this method, a known volume of the juice is mixed with an excess of iodine, which reacts with the vitamin C to form a complex. The excess iodine is then titrated with a standardized sodium thiosulfate solution, and the volume of the sodium thiosulfate used is measured. The vitamin C content of the juice can be calculated based on the volume of sodium thiosulfate used.
It is important to note that the vitamin C content of fruit juice can vary depending on factors such as the type of fruit used, the method of processing, and the storage conditions. For example, freshly squeezed orange juice may have a higher vitamin C content than store-bought orange juice that has been pasteurized and packaged in a carton. Similarly, the vitamin C content of fruit juice may decline over time due to oxidation, which can occur when the juice is exposed to air or light. To preserve the vitamin C content of fruit juice, it is recommended to store it in a cool, dark place and consume it as soon as possible after opening.
In conclusion, vitamin C is an essential nutrient that can be found in a variety of fruit juices. By analyzing the vitamin C content of fruit juice, we can determine the amount of this nutrient present and ensure that we are getting enough to support good health. However, it is important to be aware of the factors that can affect the vitamin C content of fruit juice and choose a high-quality product to get the maximum benefits.
(PDF) ANALYSIS OF VITAMIN C IN COMMERCIAL FRUIT JUICES BY IODOMETRIC TITRATION. SHAMSUL AZRIN BIN MD. KANAFE Final Year Project Report Submitted in Partial Fulfilment of the Requirement for the Degree of Bachelor of Science (Hons.) Chemistry in the
You should be able to get all the vitamin C you need for your daily diet. However, apple juice was lower amount of vitamin C because the range of vitamin C content was shorter compared mango juice and guava juice in commercial fruit juice. This is still higher than 45mg daily adult recommendation. Iodine can be decolourized by thiosulphate ion. Particularly iodometric redox titration is also used. His PSA, a measure of the cancer, had doubled…. In this case the reactant is vitamin C.
Quantitative Analysis of Vitamin C Contained in a Fruit...
Moertel CG, Fleming TR, Creagan ET, Rubin J, O'Connell MJ, Ames MM. This experiment utilized two sets of titrations utilizing separately sodium hydroxide and potassium iodate as titrant. Vitamin C is an antioxidant that helps reduce the effects of… unit 21 p2 Vitamin C — Vitamin C helps to protect cells and keep them healthy, and helps with healing wounds. Introduction Vitamin C or L-asorbic acid is commonly known as the vitamin found in citrus fruits and is known for its important role in growing and repairing tissues and ability to prevent scurvy. Generally, there are two types of titration commonly used, Acid-Base Titration, which involves neutralization reaction where the products are salt and water.
Analysis of Vitamin C Content in Processed Fruit Juices
He wrote the same book 3 times, but every time he wrote it he would up the dosage and title how it cures a bigger disease. However reaction 4 does not determine the amount of iodine produced, it only determines when iodine is present in solution. Conshoshocken, PA: Infinity Publishing; 2006. However, along with ascorbic acid, citrus fruit juices as the names indicates also contain amounts of citric acid. In 1994, he died of cancer the very thing he said Vitamin C would cure. When all available iodine has been reacted, the blue-black solution becomes colourless which signals the end-point.
Analysis of Vitamin C in Fruit opportunities.alumdev.columbia.edu
Laboratory exercise 3 : Quantitative analysis of vitamin C contained in a fruit juice Aim: To investigate the amount of vitamin C found in all kinds of fruit juice Introduction : Vitamin C is an antioxidant, along with vitamin E, beta-carotene, and many other plant-based nutrients. This sample maintained the lowest microbial count among all the stored samples and the micro-organism identified in it were mainly Saccharomyces cerevisiae and lactobacillus species. The volume of DCPIP needed for the titration process was recorded. Lastly, it helps keep your teeth and gums healthy. Hence, compare the vitamin C content in the experimental results with the manufacturer's claim and to find out the best buy. A reagent that is particularly good for the oxidation is an aqueous solution of iodine, I2. Brands Chosen Brand First Choice orange juice Qoo mango juice you.
However, I2 solution is not normally prepared directly by dissolving iodine in water because iodine is too volatile so it is almost impossible to avoid loss while the solution is being prepared. Essentially reaction 3 is used to determine how much iodine is produced. Vitamin C is particular is an L- enantiomer of ascorbic acid which is a weak acid derivative of glucose. He was the only person to receive two noble prizes by himself. Any iodine present will react with starch to form a blue-black solution.
Determination of Vitamin C in Fruit opportunities.alumdev.columbia.edu
Then the solution is titrated with DCPIP solution, until the colour change from light orange to light pink. The acid-base titration involved a reaction as shown in Figure 1 in which sodium hydroxide neutralizes all acid. In conclusion, don't stress about Vitamin C and keep drinking Orange Juice to a bear… review Vitamin C is a water soluble nutrient found in some foods. This research aims to determine the concentration of Vitamin C after heating at 80oC through a period of time by titrating the heated solution with DCPIP 2, 6-Dichloro-Indophenol solution. To add on, Vitamin C helps prevent iron deficiencies by improving the iron absorption and also by reducing the risk of.
Lamson DW, Brignall MS. Abstract This paper is a summary and analysis of the Vitamin C content in fruit juices, specifically looking at the ratio of ascorbic acid to citric acid. On the following page are the directions. It helps with the maintenance of the connective tissue, which helps to give support and structure for other tissue and organs. Both titrations were performed on orange juice and grapefruit juice separately. Next, determine the amount of fruit juice needed to decolourize 3mL of DCPIP solution, and calculating the concentration of Vitamin C in the fruit juice initially.
Another function is it increases the absorption of iron from foods. The objective of study are determination vitamin C content in three commercial fruit juices apple, guava and mango using titration method. I think I am really excited to do this project because it sounds very interesting and fun to do. Failure of high-dose vitamin C ascorbic acid therapy to benefit patients with advanced cancer. The test tubes should hold amounts of fruit juice that are more than the amount of fruit juice needed to decolourize 3mL of DCPIP initially.
In order for the reaction to undergo completion with a sharp endpoint the reagents must have a high tendency to either donate or accept electron. A titrant reagent - Dichlorophenol-indophenol of known concentration… Vitamin C Persuasive Essay Some cases Vitamin C has been proven harmful. The only reason we believe that Vitamin C is good for us in large doses is because of a man named Linus Pauling. The key in these titrations lies in the specificity to ascorbic acid as opposed to standard acid-base titration that measures the presence of all acid. To conduct an effective titration analysis, one must learn and understand the principles that is involved. Hoffer A, Pauling L.